Reverse Transcription Polymerase Chain Reaction:Article Title: Reopening the Bavarian State Opera Safely: Hygiene Strategies and Incidence of COVID-19 in Artistic Staff During Theater Season 2020/2021
Article Snippet: .. RT-PCR assays are the gold standard for SARS-CoV-2 detection with high sensitivity and specificity., The mSample Preparation System DNA kit identical to the Promega Maxwell Viral Total Nucleic Acid Extraction Kit (Promega, Medison, WI) was used for nucleic acid extraction following a standard protocol on an m2000sp device for RNA and DNA extraction (Abbott, Wiesbaden, Germany), SARS-CoV-2 RT PCR was performed using SARS-CoV-2_N1 and SARS-CoV-2_N3 primer and probe sets for amplification on an ABI 7500 real-time PCR cycler (Thermofisher Scientific, Darmstadt, Germany) following the protocol of the Division of Viral Diseases, National Center of Immunization and Respiratory Diseases, Centers for Disease Control and Prevention Atlanta, GA. Quantitative SARS-CoV-2 PCR results were calculated using a standard curve generated from 10e6, 10e4, 10e2, and 10e1 standards. ..
Extraction:Article Title: Reopening the Bavarian State Opera Safely: Hygiene Strategies and Incidence of COVID-19 in Artistic Staff During Theater Season 2020/2021
Article Snippet: .. RT-PCR assays are the gold standard for SARS-CoV-2 detection with high sensitivity and specificity., The mSample Preparation System DNA kit identical to the Promega Maxwell Viral Total Nucleic Acid Extraction Kit (Promega, Medison, WI) was used for nucleic acid extraction following a standard protocol on an m2000sp device for RNA and DNA extraction (Abbott, Wiesbaden, Germany), SARS-CoV-2 RT PCR was performed using SARS-CoV-2_N1 and SARS-CoV-2_N3 primer and probe sets for amplification on an ABI 7500 real-time PCR cycler (Thermofisher Scientific, Darmstadt, Germany) following the protocol of the Division of Viral Diseases, National Center of Immunization and Respiratory Diseases, Centers for Disease Control and Prevention Atlanta, GA. Quantitative SARS-CoV-2 PCR results were calculated using a standard curve generated from 10e6, 10e4, 10e2, and 10e1 standards. ..
DNA Extraction:Article Title: Reopening the Bavarian State Opera Safely: Hygiene Strategies and Incidence of COVID-19 in Artistic Staff During Theater Season 2020/2021
Article Snippet: .. RT-PCR assays are the gold standard for SARS-CoV-2 detection with high sensitivity and specificity., The mSample Preparation System DNA kit identical to the Promega Maxwell Viral Total Nucleic Acid Extraction Kit (Promega, Medison, WI) was used for nucleic acid extraction following a standard protocol on an m2000sp device for RNA and DNA extraction (Abbott, Wiesbaden, Germany), SARS-CoV-2 RT PCR was performed using SARS-CoV-2_N1 and SARS-CoV-2_N3 primer and probe sets for amplification on an ABI 7500 real-time PCR cycler (Thermofisher Scientific, Darmstadt, Germany) following the protocol of the Division of Viral Diseases, National Center of Immunization and Respiratory Diseases, Centers for Disease Control and Prevention Atlanta, GA. Quantitative SARS-CoV-2 PCR results were calculated using a standard curve generated from 10e6, 10e4, 10e2, and 10e1 standards. ..
Amplification:Article Title: Reopening the Bavarian State Opera Safely: Hygiene Strategies and Incidence of COVID-19 in Artistic Staff During Theater Season 2020/2021
Article Snippet: .. RT-PCR assays are the gold standard for SARS-CoV-2 detection with high sensitivity and specificity., The mSample Preparation System DNA kit identical to the Promega Maxwell Viral Total Nucleic Acid Extraction Kit (Promega, Medison, WI) was used for nucleic acid extraction following a standard protocol on an m2000sp device for RNA and DNA extraction (Abbott, Wiesbaden, Germany), SARS-CoV-2 RT PCR was performed using SARS-CoV-2_N1 and SARS-CoV-2_N3 primer and probe sets for amplification on an ABI 7500 real-time PCR cycler (Thermofisher Scientific, Darmstadt, Germany) following the protocol of the Division of Viral Diseases, National Center of Immunization and Respiratory Diseases, Centers for Disease Control and Prevention Atlanta, GA. Quantitative SARS-CoV-2 PCR results were calculated using a standard curve generated from 10e6, 10e4, 10e2, and 10e1 standards. ..
Real-time Polymerase Chain Reaction:Article Title: Reopening the Bavarian State Opera Safely: Hygiene Strategies and Incidence of COVID-19 in Artistic Staff During Theater Season 2020/2021
Article Snippet: .. RT-PCR assays are the gold standard for SARS-CoV-2 detection with high sensitivity and specificity., The mSample Preparation System DNA kit identical to the Promega Maxwell Viral Total Nucleic Acid Extraction Kit (Promega, Medison, WI) was used for nucleic acid extraction following a standard protocol on an m2000sp device for RNA and DNA extraction (Abbott, Wiesbaden, Germany), SARS-CoV-2 RT PCR was performed using SARS-CoV-2_N1 and SARS-CoV-2_N3 primer and probe sets for amplification on an ABI 7500 real-time PCR cycler (Thermofisher Scientific, Darmstadt, Germany) following the protocol of the Division of Viral Diseases, National Center of Immunization and Respiratory Diseases, Centers for Disease Control and Prevention Atlanta, GA. Quantitative SARS-CoV-2 PCR results were calculated using a standard curve generated from 10e6, 10e4, 10e2, and 10e1 standards. ..
Control:Article Title: Reopening the Bavarian State Opera Safely: Hygiene Strategies and Incidence of COVID-19 in Artistic Staff During Theater Season 2020/2021
Article Snippet: .. RT-PCR assays are the gold standard for SARS-CoV-2 detection with high sensitivity and specificity., The mSample Preparation System DNA kit identical to the Promega Maxwell Viral Total Nucleic Acid Extraction Kit (Promega, Medison, WI) was used for nucleic acid extraction following a standard protocol on an m2000sp device for RNA and DNA extraction (Abbott, Wiesbaden, Germany), SARS-CoV-2 RT PCR was performed using SARS-CoV-2_N1 and SARS-CoV-2_N3 primer and probe sets for amplification on an ABI 7500 real-time PCR cycler (Thermofisher Scientific, Darmstadt, Germany) following the protocol of the Division of Viral Diseases, National Center of Immunization and Respiratory Diseases, Centers for Disease Control and Prevention Atlanta, GA. Quantitative SARS-CoV-2 PCR results were calculated using a standard curve generated from 10e6, 10e4, 10e2, and 10e1 standards. ..
Polymerase Chain Reaction:Article Title: Reopening the Bavarian State Opera Safely: Hygiene Strategies and Incidence of COVID-19 in Artistic Staff During Theater Season 2020/2021
Article Snippet: .. RT-PCR assays are the gold standard for SARS-CoV-2 detection with high sensitivity and specificity., The mSample Preparation System DNA kit identical to the Promega Maxwell Viral Total Nucleic Acid Extraction Kit (Promega, Medison, WI) was used for nucleic acid extraction following a standard protocol on an m2000sp device for RNA and DNA extraction (Abbott, Wiesbaden, Germany), SARS-CoV-2 RT PCR was performed using SARS-CoV-2_N1 and SARS-CoV-2_N3 primer and probe sets for amplification on an ABI 7500 real-time PCR cycler (Thermofisher Scientific, Darmstadt, Germany) following the protocol of the Division of Viral Diseases, National Center of Immunization and Respiratory Diseases, Centers for Disease Control and Prevention Atlanta, GA. Quantitative SARS-CoV-2 PCR results were calculated using a standard curve generated from 10e6, 10e4, 10e2, and 10e1 standards. ..
Generated:Article Title: Reopening the Bavarian State Opera Safely: Hygiene Strategies and Incidence of COVID-19 in Artistic Staff During Theater Season 2020/2021
Article Snippet: .. RT-PCR assays are the gold standard for SARS-CoV-2 detection with high sensitivity and specificity., The mSample Preparation System DNA kit identical to the Promega Maxwell Viral Total Nucleic Acid Extraction Kit (Promega, Medison, WI) was used for nucleic acid extraction following a standard protocol on an m2000sp device for RNA and DNA extraction (Abbott, Wiesbaden, Germany), SARS-CoV-2 RT PCR was performed using SARS-CoV-2_N1 and SARS-CoV-2_N3 primer and probe sets for amplification on an ABI 7500 real-time PCR cycler (Thermofisher Scientific, Darmstadt, Germany) following the protocol of the Division of Viral Diseases, National Center of Immunization and Respiratory Diseases, Centers for Disease Control and Prevention Atlanta, GA. Quantitative SARS-CoV-2 PCR results were calculated using a standard curve generated from 10e6, 10e4, 10e2, and 10e1 standards. ..
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